Chitosan adhesives with sub-micron structures for photochemical tissue bonding

We describe a method for fabricating biocompatible chitosan-based adhesives with sub-micron structures to enhance tissue bonding. This procedure avoids coating and chemical modification of requires simple drop-casting step the adhesive film formation. Chitosan thin films (27±3 μm) were fabricated pillars (rectangular cuboid height ∼150 nm, square dimension ∼1 μm pitch ∼2 or holes (diameter ~500 nm ~1 μm, depth ~100 400 1 2 μm). Polydimethylsiloxane moulds used as negative templates solution that was cast then allowed dry form films. These applied on bisected rectangular strips small sheep intestine photochemically bonded by green laser (λ= 532 irradiance ∼110 J/cm2). The repair subsequently measured using computer-interfaced tensiometer. mould reproduced in chitosan high fidelity. achieved highest bonding strength (17.1±1.2 kPa) when compared (13.0±1.3 kPa, p<0.0001, one-way ANOVA, n=15). production patterned range demonstrated, polydimethylsiloxane single step. technique is potentially scalable produce larger surface areas.